Nicolette van Dam was born in Amsterdam in 1984. After finishing high school at the havo level at the age of 18, van Dam went to study at HBO. She didn't finish her study—instead, she was discovered and became a model. After appearing in several video clips, van Dam got the role of the conceited Bionda in the kids soap ZOOP, which was aired on the Dutch Nickelodeon. In 2005, the first movie about the show was released in Dutch theaters: Zoop in Africa. The movie was followed by Zoop in India (2006). In 2007 the film Zoop in South-America was released in the Netherlands. For the second Zoop movie, van Dam song Jadoo Jadoo, which was listed at the Kids Top 20. She was also nominated for a Golden Calf for her role in the second film.After having guest starred in the series Van Speijk in 2006, van Dam presented the reality show Pimp my Room. Her next credit as a presenter, was for the kids show Jetix Max, which is aired every Sunday on the Dutch network Jetix. van Dam also appeared in the movies Deuce Bigalow: European Gigolo and Plop in de Stad, dubbed a voice in Ice Age: The Meltdownand had a guest role in one of the most successful investigation series of the Netherlands; Baantjer. From 18 March 2007 to November 2009, van Dam had a role in the successful comedy series Voetbalvrouwen. She also take the role of Belona in Het huis Anubis Third movie called, The return of Sibuna.
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On June 18, 2014, during the 2014 FIFA World Cup, Nicolette van Dam tweeted a photo-shopped mock-up of Colombia national team members James Rodríguez and Radamel Falcao snorting vanishing foam with the caption "Colombian wall", making an allusion of snorting cocaine. The post was removed soon afterwards, followed by an apology stating "Queridos Colombianos, there was no offense intended from my side. Please accept my honest and sincere apologies". Colombian Minister of Foreign Affairs María Ángela Holguín spoke out against Van Dam, saying "This is not the first time that she has put out a disparaging tweet, and Colombia will not accept this". Holguín proceeded to request Colombian ambassador in the Netherlands, Eduardo Pizarro Leongómez, to go to the chancellery of said country in search for more severe consequences to Van Dam's "disrespect towards Colombia" As a consequence of the incident, Van Dam resigned her position as UNICEF Goodwill Ambassador